Risk waste/Risk waste cardboard boxes containing Gel-Red/Ethidium Bromide shall be marked with Gel Red/Ethidium Bromide, with name and

Description Ethidium bromide is a sensitive fluorescent stain for visualizing DNA in agarose and polyacrylamide gels. It is excited with a standard 302 nm UV transilluminator and emits a red-orange signal that can be photographed with Polaroid film or with a CCD-based gel documentation system.

Hög. 6. ≥ 480. K. 40% Natriumhydroxid. 1310-73-2. Hög. 6. ≥ 480.

Ethidium bromide should be handled in the laboratory using the "basic prudent practices" described in Chapter 5.C. Because of its mutagenicity, stock solutions of this compound should be prepared in a fume hood, and protective gloves should be worn at all times while handling this substance. Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and in cells that have compromised membranes. The fluorescence of ethidium monoazide is weak, but the intensity increases ~15-fold on binding to DNA with excitation/emiss Structure, properties, spectra, suppliers and links for: Ethidium bromide, 1239-45-8, Homidium bromide.

av J WALDENSTRÖM · 2000 · Citerat av 2 — in 2% agarose gels using 0.5xTBE buffer. The DNA was then visualised by ethidium bromide staining and UV-light. Female individuals yielded two bands.

Hög. 6. ≥ 480. 5% Ethidium bromide.

Ethidium Bromide is classified as hazardous chemical waste Solutions should be deactivated or filtered. Due to the hazardous nature of the chemicals required to deactivate the solutions, filtration is the preferred method. However, be aware that filtration will increase the concentration of the ethidium bromide. Health surveillance

SCT125 ; GelGreen is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. 2007-09-26 · More concentrated ethidium bromide. The method of choice for ethidium bromide disposal at the moment seems to be absorption onto charcoal followed by incineration. You have a 1 microgram/litre solution of ethidium bromide, a concentration that appears to be of low toxicity according to the data from cattle. Ethidium Bromide Ethidium Bromide is an intercalating agent which resembles a DNA base pair. Due to its unique structure, it can easily intercalate into DNA strand. Therefore, it is commonly used as nucleic acid fluorescent tag in various techniques of the life science field.

1g; Glass bottle. Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.15,16,17 It has been shown to bind to singlestranded DNA (although not as strongly)2 and triplestranded DNA.18 Because of the binding to DNA, EtBr is a powerful inhibitor of DNA polymerase.4 E7637, Molecular Biology grade powder, is suitable for use in gel electrophoresis and DNA isolation procedures. Ethidium bromide possesses UV absorbance maxima at 300 and 360 nm. Additionally, it can absorb energy from nucleotides excited by absorbance of 260 nm radiation. Ethidium re-emits this energy as yellow/orange light centered at 590 nm.
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The method of choice for ethidium bromide disposal at the moment seems to be absorption onto charcoal followed by incineration.

18472-51-0. Hög. 6. ≥ 480.
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Ethidium bromide is a molecule commonly used to visualize DNA in agarose gel electrophoresis experiments. It both binds to DNA and fluoresces under the proper conditions. Ethidium bromide is known

It is excited with a standard 302 nm UV transilluminator and emits a red-orange signal that can be photographed with Polaroid film or with a CCD-based gel documentation system. Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and in cells that have compromised membranes. The fluorescence of ethidium monoazide is weak, but the intensity increases ~15-fold on binding to DNA with excitation/emission maxima of ~504/600 nm. For Research Use Only.